肿瘤坏死因子-α诱导人胚肺成纤维细胞早衰的实验研究

The experimental research of premature senescence of human lung embryonic fibroblast cells induced by TNF-α

中文关键词: TNF-α  人胚肺成纤维细胞  炎性衰老  早衰

英文关键词: TNF alpha (TNF-α)  Human lung embryonic fibroblast cells  Inflamm-aging  Premature senescence

中文摘要:

      目的 研究促炎性细胞因子TNF-α是否具有诱导人胚肺成纤维细胞发生早衰的作用.方法 TNF-α连续刺激体外培养的人胚肺成纤维细胞2周,然后进行衰老相关β半乳糖苷酶染色实验,检测细胞衰老情况.结果 本研究以TNF-α作为刺激物处理人胚肺成纤维细胞,发现TNF-α可诱导细胞早衰,并建立了细胞早衰模型.结论 TNF-α具有明显的诱导人胚肺成纤维细胞衰老的作用.

英文摘要:

      Objective To explore the possible senescence-induced effect of TNF alpha (TNF-α) on human lung embryonic fibroblast cells.Methods Human lung embryonic fibroblast cells cultured in vitro were continuously treated with TNF-α for 3 weeks;senescence-associate偀馗?
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