PDGF-A基因修饰皮片对眼睑皮肤创伤修复的研究

PDGF-A gene modification in eyelid skin wound repair

中文关键词: PDGF-A基因修饰皮片  全层皮肤缺损创面  眼睑皮肤组织修复

英文关键词: Skin sheet of PDGF-A gene modification  Wound surface of full-thickness skin defect  Eyelid skin repair

中文摘要:

      目的 利用PDGF-A基因修饰的全层皮肤皮片,利用HE染色、PCR、蛋白定量分析几个层面,探讨其对眼睑皮肤损伤修复的情况及部分机理.方法 贵州小香猪6只,背部脊柱两侧造成全层皮肤缺损圆形创面(φ3.67 cm)各3个,共36个创面.A组(基因修饰皮片组)“PDGF-A基因修饰皮片”,移植到其右侧的18个创面作为实验组;B组(细胞皮片植入组)以HAM负载未经基因修饰的自体bMSCs与角朊细胞,构建成“细胞皮片”植入其左侧前6个创面;C组(单纯HAM敷盖组)单纯HAM敷盖其左侧后12个创面,作对照组.观察移植后1～4周内,各组创面愈合、肉芽组织生长、上皮化等情况.并进行创面组织HE、Laminin免疫组化及胶原含量等检测.结果 (1)A组于伤后15～17d愈合,B组于伤后21～23 d愈合,C组于伤后24 ～ 26d愈合,A组较其它两组分别提前5～6d与8～9d愈合,且愈合质量好.(2)A组创面于移植15 ～ 17 d,其肉芽组织生长旺盛,肉芽组织中Laminin、成纤维细胞和毛细血管含量丰富,可见胶原沉积.两对照组创面组织仍见许多炎细胞浸润,肉芽组织中Laminin、成纤维细胞和毛细血管含量少,胶原沉积不明显.结论 PDGF-A基因修饰皮片移植对眼睑皮肤的创伤有较好的促愈作用,愈合质量较高.

英文摘要:

      Objective To discuss PDGF-A gene modification in eyelid skin wound repair and the mechanism.Methods 6 minipigs were used in this study;3 round full-thickness wounds (φ3.68cm) were created on the dorsum of each side close to the vertebral column in each pin total,36 wounds were created;18 right side wounds were treated with skin sheet of PDGF-A gene modification as experimental group (group A),6 left side wounds were treated with human amniotic membrane (HAM) loaded withbonemarrow-derivedmesenchymalstemcells (bMSCs) and keratinocyte (group B) and 12 left side wounds were treated with HAM as control (group C);the granulation tissue,reepithelization and wound area were observed after 1,2,3 and 4 weeks;immunohistochemistry was performed using Laminin as a marker for blood vessels,as well as the detection of collagen content.Results The healing time of group A was 15 to 17 days,that of group B was 21 to 23 days and that of group C was 24 to 26 days;the healing time of group A was 5 to 6 days faster than that of group B and 8 to 9 days faster than that of group C;15 to 17 days after the graft,the granulation tissue,which contained a lot of Laminin,fibroblasts,capillaries and collagen,grew quite well in group A while many inflammatory cells were still seen in group B and group C,and the contents of Laminin,fibroblasts,capillaries and collagen in granulation tissue were smaller than those in group A.Conclusions The graft of skin sheet of PDGF-A gene modification is of good healing effect on eyelid skin wounds,the wounds heal with high quality.?飼?