肝细胞生长因子对紫外线诱导皮肤成纤维细胞产生活性氧的影响

Effect of Hepatocyte Growth Factor on Reactive Oxygen Species in UVA-induced Skin Fibroblasts

中文关键词: HGF  光老化  成纤维细胞  ROS

英文关键词: hepatocyte growth factor (HGF)  photoaging  fibroblasts (FBs)  reactive oxygen species (ROS)

中文摘要:

      目的 探讨HGF对紫外线诱导成纤维细胞产生ROS的影响.方法 采用cck-8法检查不同浓度HGF对成纤维细胞(FBs)活性的影响.HGF预处理FBs 2h,装载ROS检测探针,UVB照射诱导体外成纤维细胞发生氧化应激,荧光显微镜、酶标仪分别检测ROS的表达.结果 5ng/ml HGF对成纤维细胞的增值无明显影响.在UVB诱导的应激性老化中,ROS表达显著升高(P＜0.01).5ng/ml HGF能显著降低UVB引起的ROS表达(P＜0.05).结论 HGF可降低UVB造成的光老化FBs中ROS的表达,减少氧化应激反应,可作为对抗光老化的新手段.

英文摘要:

      Objective To explore the effect of hepatocyte growth factor (HGF) on reactive oxygen species (ROS) in UVA-induced skin fibroblasts.Methods The effect of HGF of different dosage on the activity of fibroblasts (FBs) was detected by cck-8 assay;after HGF pretreatment for 2 hours,ROS test probe was loaded and oxidative stress of FBs was induced by UVB irradiation in vitro;the expression of ROS was measured by fluorescence and enzyme-labeling instrument respectively.Results 5ng/ml HGF had no obvious effect on FBs proliferation;the expression of ROS increased greatly in UVB-induced oxidative stress (P＜0.01);5ng/ml HGF reduced the ROS expression caused by UVB induction (P＜0.05).Conclusions HGF can reduce ROS expression in FBs induced by UVB irradiation and decrease oxidative stress,it can be taken as a new anti-photoaging measure.