红景天苷对Aβ1-40诱导PC12细胞损伤的保护作用及机制

Protective Effect of Salidroside on PC12 Cell Injury Induced by Aβ1-40 and the Mechanism

中文关键词: β-淀粉样蛋白1-40  阿尔茨海默病  红景天苷  三磷酸腺苷

英文关键词: Aβ1-40  Alzheimer's disease  salidroside  adenosine triphosphate (ATP)

中文摘要:

      目的 观察红景天苷对Aβ1-40诱导PC12细胞损伤的保护作用,探讨改善能量代谢障碍对阿尔茨海默病发生发展的干预机制.方法 利用Aβ140诱导PC12细胞损伤模型,采用CCK-8法检测细胞活力,ATP试剂盒(比色法)检测ATP含量,免疫荧光法和Western blot法检测相关蛋白PGC-1α的表达.结果 红景天苷能够抑制Aβ1-40诱导的PC12细胞活力下降(P＜0.05),增加ATP含量(P＜0.01),免疫荧光和Western blot结果均显示红景天苷能够上调PC12细胞的PGC-1α蛋白表达(P＜0.05).结论 红景天苷可能通过上调能量代谢相关蛋白PGC-1a的表达,从而提高ATP含量来抑制Aβ140诱导PC 12细胞的损伤,对阿尔茨海默病的治疗具有一定的应用价值.

英文摘要:

      Objective To observe the effect of salidroside on PC12 cell injury induced by Aβ1-40 and to explore its mechanism of improving energy metabolism disorder in the development of Alzheimer's disease.Methods PC12 cells were treated with Aβ1-40to build an AD cell model;CCK-8 method was applied in the measurement of cell viability and an ATP assay kit in the measurement of adenosine triphosphate (ATP) level;the expression level of PGC-1α was detected via immunofluorescence assay and Western blot analysis.Results Salidroside inhabited the decrease of PC 12 cell viability induced by Aβ1.40 (P＜0.05) and increased the level of ATP (P＜0.01);the results of innunofluorescence assay and Western blot analysis indicated that salidroside up-regulated the expression of PGC-1α (P＜0.05).Conclusions Salidroside may inhibit Aβ1-40-induced PC12 cells injury by up-regulating the expression of PGC-1α,an energy metabolism related protein,and increasing the level of ATP,it may be of certain curative effect on Alzheimer's disease.