雷帕霉素抑制光老化成纤维细胞周期阻滞的研究

Inhibiting Effect of Rapamycin on the Cell Cycle Arrest of Photoaged Fibroblasts

中文关键词: 雷帕霉素  光老化  成纤维细胞  周期阻滞

英文关键词: rapamycin  photoaging  fibroblast  cycle arrest

中文摘要:

      目的 探讨雷帕霉素(rapamycin,RAPA)对光老化成纤维细胞(fibroblasts,FBs)周期及相关蛋白的影响.方法 采用CCK-8法检测不同浓度的RAPA对FBs活性的影响,甄选最佳应用浓度.利用UVB体外反复照射构建FBs的老化模型,流式细胞技术榆测各组周期的分布变化,Western Blot技术检测细胞周期相关蛋白p53和p21的表达.结果 低浓度RAPA对细胞活性并无影响.与UVB照射组比较,RAPA+ UVB组的S期细胞比例下降了6.5％,细胞周期相关蛋白p53和p21的表达分别下降了26.3％和27.8％.结论 RAPA可以缓解光老化FBs的S期阻滞,降低周期蛋白p53和p21的产生,一定程度上改善UVB照射导致的细胞衰老.

英文摘要:

      Objective To explore the effect of rapamycin (RAPA) on the cell cycle of photoaged fibroblasts (FBs) and on the expressions of associated proteins.Methods Cell counting kit-8 (CCK-8) assay was applied in detecting the effect of RAPA of different concentration on the activity of FBs so as to find out the most suitable concentration;the aged model of FBs was built up by repeated UVB radiation;flow cytometry was applied in detecting the changes of cell cycle distribution while Western Blot Analysis in detecting the expressions of proteins related to cell cycle:protein p53 and protein p21.Results RAPA of low concentration was of no effect on cell activity;compared with FBs radiated by UVB,the cell ratio in S phase of FBs pretreated by RAPA and UVB decreased 6.5％,the expressions of protein p53 and protein p21 decreased 26.3％ and 27.8％ respectively.Conclusions RAPA can relieve the S-phase arrest of photoaged FBs,decrease the expression of protein p53 and protein p21,and thus improve the cell aging induced by UVB radiation to some extent..