芪防膝痹颗粒治疗膝骨关节炎的机制研究

Mechanism of Qifangxibi Granule in the treatment of Knee Osteoarthritis

中文关键词: 骨关节炎  膝  骨和骨组织  细胞凋亡  芪防膝痹颗粒

英文关键词: osteoarthritis  knee  bone and bones  apoptosis  Qifangxibi Granule

中文摘要:

      目的 观察芪防膝痹颗粒对膝骨关节炎大鼠软骨组织形态及软骨细胞凋亡的影响.方法 将60只雄性SD大鼠随机分为对照组(N)、生理盐水组(S)、氨基葡萄糖组(A)、芪防膝痹颗粒低剂量组(D)、芪防膝痹颗粒中剂量组(Z)和芪防膝痹颗粒高剂量组(G)6组,每组10只.N组只打开关节腔,其余各组制备膝骨关节炎模型.8周后N组和S组予生理盐水灌胃,A组予硫酸氨基葡萄糖灌胃;D组、Z组及G组每H予1/2大鼠剂量、1倍大鼠剂虽和2倍大鼠剂量的芪防膝痹颗粒溶液灌胃.灌胃8周后取右膝关节行Micro-CT扫描,切片后行HE、阿尔辛兰/橙黄G染色、免疫组化及TUNEL染色,采用Mankin's评分法进行评分.结果 Micro-CT、HE、阿尔辛兰结果显示芪防膝痹颗粒各组均可抑制软骨退变,G组、Z组Manikin's评分均较S组低(P＜0.05);A组与G组、Z组效果相当.与S组比较,G组、Z组膝关节软骨细胞凋亡蛋白Bax、Fas的表达降低(P＜0.05);抑制凋亡的Bcl-2在G组、Z组及A组表达增高(P＜0.05).结论 芪防膝痹颗粒可以通过抑制软骨细胞凋亡来延缓膝关节软骨的退变.

英文摘要:

      Objective To observe the effect of Qifangxibi Granule on the cartilage histomorphology and chondrocyte apoptosis in rats with knee osteoarthritis (KOA).Methods 60 male SD rats were randomly divided into 6 groups:group N (normal control),group S (normal saline control group),group A (glucosamine),group D (low-dosage of QifangxibiGrandule),groupZ (middle-dosageofQifangxibiGrandule) andgroupG (high-dosageofQifangxibiGrandule),10 rats in each;the articular cavities of the rats in group N were exposed and KOA modals were prepared in other groups;8 weeks later,gastric perfusion with saline was performed on rats in group N and group S and that of glucosamine sulfate was performed on rats in group A;Qifangxibi Grandule solution with 1/2 of normal rat dosage,with 1 time of normal dosage and with 2 times of normal dosage were applied,by gavage,to rats in group D,group Z and group G every day,respectively;8 weeks after gavage,the left knee joints were collected and Micro-CT scanning was made;after section,HE staining,ABHO/G staining,immunohistochemical assay and TUNEL were performed,and Mankin's scale was then made.Results The results of Micros-CT,HE staining and ABHO/G staining indicated that Qifangxibi Grandule was of certain effect in inhibiting the cartilage degeneration in each group,the Mankin's scores in group G and group Z were lower than those in group S (P＜0.05);the result in group A was the same with those in group G and group Z;the expressions of protein Bax and Fas in chondrocyte apoptosis in group G and group Z decreased much more than those in group S (P＜0.05);the expression of Bcl-2 which controlled the apoptosis increased in group G,group A and group A (P＜0.05).Conclusions Qifangxibi Granule may be effective in delaying the cartilage degeneration through inhibiting chondrocyte apoptosis.