同源盒基因A10表达下调后抑制胃癌细胞裸鼠皮下成瘤能力及相关机制研究

HOXA10 with Down-regulated Expression in Inhibiting Xenograft Tumor Formation of Gastric Cancer Cell and the Mechanism

中文关键词: 胃肿瘤  同源盒基因A10  裸鼠皮下成瘤  B细胞淋巴瘤/白血病-xL

英文关键词: stomach neoplasms  homeobox gene A10 (HOXA10)  xenograft tumor formation  Bcl-xL

中文摘要:

      目的 探讨同源盒基因A10(homeobox gene A10,HOXA10)表达水平改变后对胃癌细胞裸鼠皮下成瘤能力的影响及其可能涉及的机制.方法 利用生物信息学方法分析The Cancer Genome Atlas website (TCGA)数据库中HOXA 10在胃癌中的表达水平,构建HOXA 10表达降低稳转胃癌细胞株BGC-823-sh-HOXA 10并进行裸鼠皮下成瘤实验,采用western blot检测HOXA 10敲减后凋亡相关蛋白B细胞淋巴瘤/白血病-xL(Bcl-xL)表达水平的改变情况.结果 分析TCGA数据库后发现胃癌中HOXA 10表达水平升高.HOXA 10表达下调后,胃癌细胞株BGC-823裸鼠皮下成瘤能力减弱,凋亡相关蛋白Bcl-xL表达下调.结论 HOXA 10在胃癌中表达异常升高,HOXA 10表达水平降低后可抑制胃癌细胞BGC-823裸鼠皮下成瘤能力,影响抗凋亡蛋白Bcl-xL表达水平可能是其发挥作用的机制.

英文摘要:

      Objective To investigate the effect of homeobox gene A10 (HOXA10) with changed expression level on xenograft tumor formation of gastric cancer cell and the possible mechanism.Methods Bioinformatics analysis was made to the expression of HOXA10 in gastric cancer in The Cancer Genome Atlas website (TCGA) database;stable HOXA10 knockdown gastric cancer cell line BGC-823-sh-HOXA10 was constructed and performed in the xenograft tumor formation assay;the expression of apoptosis related protein Bcl-xL was detected by western blot.Results The analysis of TCGA database showed that the expression of HOXA 10 in gastric cancer was up-regulated;HOXA10 knockdown impaired the ability of xenograft tumor formation in BGC-823 cell line and the expression of Bcl-xL was down-regulated.Conclusions HOXA10 was upregulated in gastric cancer;HOXA10 knockdown may inhibit the ability of xenograft tumor formation in BGC-823 cell line and the down-regulation of antiapoptosis protein Bcl-xL after HOXA10 knockdown may be the possible mechanism.