miRNA-223在老年人非酒精性脂肪肝患者中的表达变化及诊断价值

The expression changes and diagnostic value of miRNA-223 in elderly patients with non-alcoholic fatty liver disease

中文关键词: miRNA-223  非酒精性脂肪肝  老年

英文关键词: microRNA-223  non-alcoholic fatty liver disease  elderly

中文摘要:

      目的 探讨血清微小RNA-223(miR-223)在老年人非酒精性脂肪肝(NAFLD)中的临床应用价值和诊断价值,为早期诊断老年NAFLD提供依据.方法 收集NAFLD病例80 例以及同期体检的非NAFLD者80 例为对照组(年龄≥65 岁).检测血清生化指标以及CAP值.实时定量PCR法检测两组血清miR-223 的表达水平.Pearson直线相关分析法分析老年NAFLD患者miR-223 与肝功能、血脂以及脂肪肝程度的相关性.ROC曲线下面积(AUC)评估血清miR-223对老年NAFLD的诊断价值.结果 与对照组比较,老年NAFLD组血清ALT,GGT,TG,Ch,FBG水平以及CAP值高于对照组(P＜0.05).NAFLD组血清miR-223 水平增高(P＜0.05).Pearson相关性结果提示,老年NAFLD患者miR-223 水平与TG、LDL、HDL呈正相关性(P＜0.05).ROC曲线分析结果显示,miR-223 诊断NAFLD的AUC为 0.809(P =0.001).结论 miR-223 高表达于老年NAFLD患者的外周循环血清中,miR-223 的表达与TG、LDL、HDL呈正相关性.miR-223 可能成为早期诊断老年人非酒精性脂肪肝的潜在标志物.

英文摘要:

      Objective To investigate the clinical application and diagnostic value of serum microRNA?223 (miR?223) in the elderly with non?alcoholic fatty liver disease (NAFLD), and provide evidence for the early diagnosis of NAFLD in the elderly. Methods 80 NAFLD patients (NAFLD group) and 80 subjects (control group) without NAFLD who underwent physical examination during the same period were enrolled, aged ≥65 years. Their serum biochemical indexes and CAP values were detected. The expression level of serum miR?223 of the two groups was detected by real?time quantitative PCR. Pearson linear correlation analysis was used to analyze the correlation between miR?223 and liver function, blood lipids, and severity of fatty liver in elderly NAFLD patients. The area under ROC curve (AUC)was used to evaluate the diagnostic value of serum miR?223 in elderly patients with NAFLD. Results The levels of serum ALT, GGT, TG, Ch and FBG and CAP values of the NAFLD group were higher than those of the control group (P <0. 05). Compared with the control group, the serum miR?223 level of the NAFLD group increased (P <0. 05). Pearson correlation results indicated that miR?223 levels were positively cor? related with the level of TG, LDL and HDL in elderly NAFLD patients (P <0. 05). The result of ROC curve showed that the area under the curve (AUC) of miR?223 in the diagnosis of NAFLD was 0. 809 (P =0. 001). Conclusion miR?223 is highly expressed in the peripheral circulating blood of elderly NAFLD patients, and the expression of miR?223 is positively correlated with TG, LDL, and HDL. miR?223 may be a potential marker for early diagnosis of NAFLD in the elderly.