Lemur酪氨酸激酶3在老年肺腺癌患者肺腺癌组织中的表达及临床意义

Expression and clinical significance of Lemur tyrosine kinase 3 in lung adenocarcinoma tissues of elderly patients

中文关键词: 老年  肺癌  Lemur酪氨酸激酶3  TNM分期  淋巴结转移

英文关键词: elderly  lung cancer  Lemur tyrosine kinase-3  TNM staging  lymph node metastasis

中文摘要:

      目的 探讨Lemur酪氨酸激酶3(LMTK3)在老年肺腺癌患者组织和血清中的表达,及LMTK3 差异表达与患者临床特征的关系.方法 选取2021 年1 月—2022 年12 月在上海市第六人民医院金山分院诊治的老年肺腺癌患者,收集肺腺癌患者手术切除标本10 例,免疫组化检测肺腺癌组织和癌旁组织中LMTK3 蛋白的表达,计算阳性细胞的表达百分率.收集肺腺癌患者外周血清86 例,同期老年健康体检者外周血清 80 例作为对照组,ELISA法检测血清中LMTK3 水平,分析LMTK3 水平与肺腺癌患者临床特征的关系.结果 在老年肺腺癌及其癌旁正常组织内均检测到LMTK3 蛋白的存在,并且在癌组织中LMTK3 的蛋白水平明显高于癌旁正常组织,具有统计学差异(P＜0.05).肺腺癌患者血清中LMTK3 水平明显高于正常对照组[(16.35±3.41)ng/mL vs(4.70±2.05)ng/mL](P＜0.05),而且与更高的TNM分期(P＜0.05)和淋巴结转移(P＜0.05)相关.结论 LMTK3 在老年肺腺癌组织和血清中呈高表达,并可能与较高的TNM分期及淋巴结转移情况密切相关.

英文摘要:

      Objective To investigate the expression of Lemur tyrosine kinase 3(LMTK3)in the tissues and serum of elderly patients with lung adenocarcinoma,and study the relationship between the differential expression of LMTK3 and the clinical characteristics of patients.Methods Elderly patients with lung adenocarcinoma diagnosed and treated in Jinshan Branch of Sixth People's Hospital of Shanghai City from January 2021 to December 2022 were selected.Surgical resection specimens from 10 patients with lung adenocarcinoma were collected.The expression of LMTK3 protein in lung adenocarcino-ma tissues and adjacent tissues were detected by immunohistochemical method,and the expression percentage of positive cells was calculated.The peripheral blood serum samples from 86 patients with lung adenocarcinoma were collected,and another pe-ripheral serum samples from 80 elderly healthy subjects who underwent physical examinations during the same period were col-lected as the control group.The level of serum LMTK3 were detected by ELISA,and the relationship between LMTK3 level and clinical features of patients with lung adenocarcinoma was analyzed.Results LMTK3 protein was detected in both lung ad-enocarcinoma tissue and adjacent normal tissues,and the level of LMTK3 protein in the cancerous tissues was significantly higher than that in the adjacent normal tissues,with statistical difference(P＜0.05).The level of serum LMTK3 in the lung adenocarci-noma patients was significantly higher than that in the control group[(16.35±3.41)ng/mL vs(4.70±2.05)ng/mL](P＜0.05),and higher LMTK3 level was associated with higher TNM staging(P＜0.05)and lymph node metastasis(P＜0.05).Conclusion LMTK3 is highly expressed in lung adenocarcinoma tissues and serum of elderly patients with lung adenocarcino-ma,which may be closely related to higher TNM staging and lymph node metastasis.