基于孟德尔随机化分析方法探求肌酐与椎间盘退行性疾病的相关性

An analysis based on the Mendelian randomization method exploring the correlation between creatinine and degenerative inter-vertebral disc disease

中文关键词: 椎间盘退行性疾病  肌酐  孟德尔随机化

英文关键词: degenerative intervertebral disc disease  creatinine  mendelian randomization

中文摘要:

      目的 运用孟德尔随机化模型探索肌酐水平对椎间盘退行性疾病的影响.方法 利用双向孟德尔随机化方法,评估肌酐水平与椎间盘退行性疾病之间的相关性.采用两个独立的GWAS公共遗传数据库,在孟德尔随机化分析中,以逆方差加权法的结果作为主要评价指标,另外4种方法作为参考;CochransQ异质性检测、孟德尔随机化Egger-intercept检测和孟德尔随机化-PRESSO法用于检测异质性和多效性;留一法、敏感性森林图、漏斗图用于检测敏感性;Radial-孟德尔随机化法用于检测异常值.结果 肌酐水平对椎间盘退行性疾病存在显著的正向因果效应[逆方差加权法:OR=1.218;95％CI:(1.085,1.368);P=0.001,＜0.05].椎间盘退行性疾病对肌酐不存在因果效应[逆方差加权法:OR=0.961;95％CI:(0.923,1.001);P=0.055,＞0.05],异质性检测表明无显著偏倚存在(P=0.081,＞0.05),水平多效性检测显示低多效性偏倚(P=0.471,＞0.05).结论 肌酐水平升高是椎间盘退行性疾病的潜在不利因素.

英文摘要:

      Objective Utilizing the Mendelian randomization model to explore the impact of creatinine levels on Degen-erative intervertebral disc disease.Methods Using bidirectional Mendelian randomization,this section evaluates the correla-tion between creatinine levels and Degenerative intervertebral disc disease.This study employed two independent public genetic databases from Genome-Wide Association Studies(GWAS),this study primarily evaluates the causal relationship using the re-sults of the Inverse-variance weighted method in Mendelian randomization analysis,with four additional methods serving as ref-erences.CochransQ test for heterogeneity,Mendelian randomization Egger-intercept test,and Mendelian randomization-PRES-SO method employed for detecting heterogeneity and pleiotropy;Leave-one-out analysis and sensitivity,with forest plots and fun-nel plots utilized for sensitivity detection;Radial-Mendelian randomization approach used for outlier detection.Results Creati-nine levels showed a significant positive causal effect on Degenerative intervertebral disc disease[IVW:OR=1.218;95％CI:(1.085,1.368);P=0.001,＜0.05].Degenerative intervertebral disc disease has no causal effect on creatinine levels[IVW:OR=0.961;95％CI:(0.923,1.001);P=0.055,＞0.05].Heterogeneity tests indicated no significant bias(P=0.081＞0.05),and Pleiotropy tests showed a low level of bias(P=0.471,＞0.05).Conclusion Elevated creatinine levels are a potential risk factor for degenerative intervertebral disc disease.