哮喘对膝骨关节炎影响的孟德尔随机化研究

Mendelian randomization study on effects of asthma on knee osteoarthritis

中文关键词: 哮喘  膝骨关节炎  孟德尔随机化  单核苷酸多态性

英文关键词: asthma  knee osteoarthritis  Mendelian randomization  single-nucleotide polymorphism

中文摘要:

      目的 利用孟德尔随机化(MR)研究分析哮喘对膝骨关节炎(KOA)的影响.方法 以逆方差加权法(IVW)作为主要评估方法,同时采用MR-Egger回归、加权中位值(WM)、基于众数的简单估计和基于众数的加权估计4种方法检验结果的可靠性与稳定性.留一法对研究结果进行敏感性分析.Cochran's Q检验计算截距和P值,分析IVW与MR-Egger 各SNP的异质性.MR-pleiotropy函数对研究结果进行多效性分析.两样本MR评估哮喘(主数据集和次要数据集)与KOA的因果关系,反向MR研究KOA对哮喘的影响.结果 哮喘主数据集与KOA的发生概率增加有关(OR=1.664,95％CI:1.134～2.441,P=0.009).哮喘次要数据集与KOA的发生概率增加有关(OR=6 405.249,95％CI:1.850～22 176 818.850,P=0.035).留一法显示正向MR结果稳定.反向MR研究显示,KOA与哮喘的发生概率增加无关(P＞0.05).结论 哮喘一定程度上能够促进KOA的发生和发展,是KOA的潜在不利因素.

英文摘要:

      Objective To investigate the effects of asthma on knee osteoarthritis(KOA)by means of Mendelian ran-domization(MR)method.Methods Inverse variance weighting(IVW)was used as the main evaluation method.At the same time,four methods were used to test the reliability and stability of the results:MR Egger regression,weighted median(WM),simple estimation based on mode,and weighted estimation based on mode.Leave-one-out method was used for sensi-tivity analysis of research results.Cochran's Q-test was used to calculate intercept and P value,and analyze the heterogeneity of SNP of IVW and MR-Egger results.The MR-pleiotropy function was used to analyze the research results.Two sample MR as-sessments were conducted to assess the causal relationship between asthma(main datasets and secondary datasets)and KOA,and reverse MR were conducted to investigate the effects of KOA on asthma.Results The asthma main dataset was associated with an increased probability of KOA occurrence(OR=1.664,95％CI:1.134-2.441,P=0.009).The asthma secondary datasets were associated with an increased probability of KOA occurrence(OR=6 405.249,95％CI:1.850-22 176 818.850,P=0.035).The results of leave-one-out method showed that the positive MR result was stable.Reverse MR studies showed that KOA was not associated with an increased probability of asthma occurrence(P＞0.05).Conclusion Asthma can pro-mote the occurrence and development of KOA to a certain extent,and is a potential adverse factor for KOA.