老年慢性便秘患者肠道菌群特点的研究

Characterization of gut microbiota in elderly patients with chronic constipation

中文关键词: 老年  慢性便秘  肠道菌群  16srRNA基因测序

英文关键词: elderly  chronic constipation  gut microbiota  16s rRNA gene sequencing

中文摘要:

      目的 对老年慢性便秘人群的肠道菌群特点进行初步探索.方法 纳入2018年1月 2018年3月期间在复旦大学附属华东医院就诊的89例老年慢性便秘患者作为老年慢性便秘组,并选取20名健康老年人作为健康对照组,采集其粪便样本,运用16s rRNA高通量测序技术进行肠道菌群特征分析.数据分析使用STATISTICA 10.0软件,根据数据类型分别采用t检验及x2检验进行组间比较.结果 老年慢性便秘患者的肠道菌群组成与健康老年人相比存在显著差异.Beta多样性分析表明,2组菌群组成不同,老年便秘患者的肠道菌群呈现出丰富度和多样性均降低的特征.老年便秘组的 Anaerostipes、Bacteroides、Blautia、Faecalibacterium、Lachnospira、Megamonas等属相对丰度明显更低(0.10％vs 0.62％;42.28％vs46.58％;0.33％vs 1.30;3.89％vs 6.10％;1.04％vs 1.92％;1.26％vs1.51％);而其他一些细菌属在老年慢性便秘组中相对丰度更高,如 Dialister、Escherichia、Prevotella、Ruminiclostridium、Ruminococcus 等(1.05％vs 0.69％;0.89％vs0.17％;7.29％vs 5.72％;0.98％vs0.32％;1.77％vs0.92％).在种的水平上,老年便秘组相对丰度占优势的物种有3个,分别为s__Bacteroides_coprophilus、g__Holdemanella、g__Anaerotruncus.相比之下,健康对照组有12个,其中5个属于毛螺菌科,3个属于颤螺菌科,1个属于月形单胞菌科.结论 老年慢性便秘患者的肠道菌群结构发生了显著变化,本研究为进一步研究老年慢性便秘的发病机制及探索潜在治疗策略提供了重要依据.

英文摘要:

      Objective To preliminarily explore the characteristics of gut microbiota in elderly patients with chronic con-stipation.Methods A total of 89 elderly patients with chronic constipation treated in Huadong Hospital Affiliated to Fudan University from January 2018 to March 2018 were included as the elderly chronic constipation group,and 20 healthy elderly people were selected as the healthy control group.Their stool samples were collected,and 16s rRNA high-throughput sequen-cing technology was used to analyze the characteristics of gut microbiota.Data were analyzed by STATISTICA 10.0 software,and inter-group comparisons were conducted by t-tests and x2 tests,respectively,according to data types.Results There was a significant difference in the composition of gut microbiota between the two groups.Beta diversity analysis showed that the composition of the gut microbiota of the two groups was different,and richness and diversity of gut microbiota in the elderly constipation group decreased.The relative abundance of Anaerostipes,Bacteroides,Blautia,Faecalibacterium,Lachnospira and Megamonas in the elderly constipation group was significantly lower(0.10％vs 0.62％;42.28％vs 46.58％;0.33％vs 1.30;3.89％vs 6.10％;1.04％vs 1.92％;1.26％vs 1.51％).However,other bacterial genera showed relative abundance in the elderly chronic constipation group were higher,such asDialist,Escherichia,Prevotella,Ruminiclostridium,Ruminococ-cus,etc.(1.05％vs 0.69％;0.89％vs 0.17％;7.29％vs 5.72％;0.98％vs 0.32％;1.77％vs0.92％).At the species level,three species were predominant in the elderly constipation group,including s Bacteroides_coprophilus,g Holdemanella,and g Anaerotruncus.In contrast,the healthy control group had 12 predominant species,including five belonging to the Muri-baculaceae family,three to the Rikenellaceae family,and one to the Lachnospiraceae family.Conclusion The gut microbiota composition in elderly patients with chronic constipation has undergone significant changes.This study provides an important basis for further studying the pathogenesis of chronic constipation in the elderly and exploring potential treatment strategies.