lncRNA SNHG12在老年乳腺癌中的表达及其与临床病理参数、预后的相关性分析

Expression of lncRNA SNHG12 in breast cancer tissues of elderly patients and its correlation with clinicopathological parame-ters and prognosis

中文关键词: 老年  乳腺癌  长链非编码RNA SNHG12  临床病理特征  预后

英文关键词: elderly  breast cancer  long non-coding RNA SNHG12  clinicopathological characteristic  prognosis

中文摘要:

      目的 分析长链非编码RNA小核仁RNA宿主基因12(lncRNA SNHG12)在老年乳腺癌中的表达及其与临床病理参数、预后的相关性.方法 选取2019年4月—2021年5月焦作市人民医院行乳腺癌手术患者标本76例,包括癌组织和癌旁组织(癌周5 cm范围).实时荧光定量聚合酶链式反应(RT-PCR)检测癌组织和癌旁组织中lncRNA SNHG12水平,分析lncRNA SNHG12与乳腺癌临床病理参数的关系;患者均进行3年有效随访,统计肿瘤复发、转移及患者死亡情况;采用Kaplan-Meier生存曲线分析lncRNA SNHG12差异表达对老年乳腺癌患者预后的影响;采用Cox回归分析老年乳腺癌预后的相关因素.结果 癌组织中lncRNA SNHG12相对表达量高于癌旁组织[(1.98±0.23),vs(0.65±0.07),t=48.227,P＜0.05].lncRNA SNHG12在老年乳腺癌中表达水平与肿瘤最大径、淋巴结转移和ER有关(P＜0.05).Cox回归分析结果显示,肿瘤最大径＞2 cm(OR=3.337,95％CI=1.167～9.541)、有淋巴结转移(HR=3.031,95％CI=1.282～7.167)、ER 阴性(HR=4.702,95％CI=1.098～20.132)、lncRNA SNHG12 高表达(HR=3.762,95％CI=1.339～10.569)是影响患者预后的独立危险因素(P＜0.05).lncRNA SNHG12高表达组3年生存率低于低表达组(70.59％vs 90.48％)(Log-rankx2=5.614,P=0.017).结论 老年乳腺癌组织中lncRNA SNHG12表达可能高于癌旁组织,lncRNA SNHG12差异表达与多种临床病理参数可能有关,可望作为老年乳腺癌患者不良预后的重要指标.

英文摘要:

      Objective To analyze the expression of long non-coding RNA small nucleolar RNA host gene 12(lncRNA SNHG12)in breast cancer tissues of elderly patients and its correlation with clinicopathological parameters and prognosis.Methods A total of 76 specimens of breast cancer tissue and paired adjacent tissues(within 5 cm of the tumor margin)were collected from elderly patients who underwent breast cancer surgery in People's Hospital of Jiaozuo City between April 2019 and May 2021.Real-time quantitative polymerase chain reaction(RT-PCR)was used to detect lncRNA SNHG12 levels in cancer and adjacent tissues.The relationship between lncRNA SNHG12 expression and clinicopathological parameters was analyzed.All patients were effectively followed up for 3 years.The recurrence,metastasis and death of patients were analyzed.Kaplan-Meier survival curves were used to assess the effects of lncRNA SNHG12 differential expression on prognosis,and Cox regres-sion analysis was performed to identify prognostic factors.Results The relative expression of lncRNA SNHG12 was signifi-cantly higher in cancer tissues than that in adjacent tissues[(1.98±0.23)vs(0.65±0.07),t=48.227,P＜0.05].The ex-pression level of lncRNA SNHG12 was correlated with maximum tumor diameter,lymph node metastasis,and estrogen receptor(ER)(P＜0.05).Cox regression analysis showed that maximum tumor diameter＞2 cm(HR=3.337,95％CI=1.167-9.541),lymph node metastasis(HR=3.031,95％CI=1.282-7.167),ER negativity(HR=4.702,95％CI=1.098-20.132),and high expression of lncRNA SNHG12(HR=3.762,95％CI=1.339-10.569)were independent risk factors for poor prognosis(P＜0.05).The 3-year survival rate was lower in the lncRNA SNHG12 high-expression group than that in the low-expression group(70.59％vs 90.48％;Log-rank x2=5.614,P=0.017).Conclusion The expression of lncRNA SNHG12 in breast cancer tissues of elderly patients may be higher than that in adjacent tissues.The differential expression of ln-cRNA SNHG12 may be related to a variety of clinicopathological parameters,and may be used as an important indicator for poor prognosis in elderly breast cancer patients.