不同化疗阶段老年食管癌患者肠道菌群的变化

Changes in gut microbiota of elderly patients with esophageal cancer at different stages of chemotherapy

中文关键词: 老年  食管癌  肠道微生物  一期化疗  二期化疗  粪便  ROC曲线

英文关键词: elderly  esophageal cancer  gut microbiota  primary chemotherapy  secondary chemotherapy  faeces  re-ceiver operating characteristic curve

中文摘要:

      目的 本研究通过肠道菌群多样性比较了化疗对老年食管癌患者肠道菌群影响,并揭示不同化疗阶段食管癌患者潜在的菌群差异.方法 选取2024年3月-2024年5月河南省南阳市中心医院收治的食管癌患者与体检健康者,共收集44份样本,其中健康者粪便11份,食管癌患者治疗前粪便11份,患者第一次化疗后粪便11分,患者第二次化疗后粪便11份,分别计为Cont组、ESCC-0、ESCC-1和ESCC-2组.分析4组肠道菌群的组成及差异变化.结果 通过16S rRNA基因测序技术,对患者的肠道菌群进行了全面分析,正常人群中g_Bacteroides、g_Megamonas和g_Para-bacteroides贡献度较高,这些菌群在维持肠道菌群稳态中发挥重要作用.在第一次化疗后,c_Betaproteobacteria、o_Burkholderiales和g_Burkholderia的贡献度上升,反映出化疗对肠道菌群的影响.而在第二次化疗后,f_Lactobacil-laceae、g_Eubacterium和g_Lactobacillus菌群的贡献度增加,提示肠道微环境的修复与免疫调节的恢复、肠道菌群的动态变化与食管癌的发展及化疗反应密切相关.结论 食管癌患者和正常人群的菌群构成存在显著差异,化疗次数的增加对食管癌患者的肠道菌群的多样性和菌群结构产生影响.部分菌群可能作为潜在的诊断标志物和治疗靶点,为食管癌的精准医疗提供新思路.

英文摘要:

      Objective This study compared the effects of chemotherapy on gut microbiota diversity in elderly esophage-al cancer patients and revealed potential microbiota differences at different stages of chemotherapy.Methods Patients with e-sophageal cancer admitted to Central Hospital of Nanyang City and healthy individuals undergoing physical examinations in the same hospital from March 2024 to May 2024 were selected.A total of 44 fecal samples were collected.Among them,there were 11 fecal samples from healthy individuals,11 ones from esophageal cancer patients before treatment,11 ones from pa-tients after their first chemotherapy,and 11 ones from patients after their second chemotherapy.They were set as Cont group,ESCC-0 group,ESCC-1 group and ESCC-2 group,respectively.The composition and variation of gut microbiota were ana-lyzed and compared among the four groups.Results The gut microbiota of the patients was comprehensively analyzed through 16S rRNA gene sequencing technology.In the normal population,the contribution of g_Bacteroides,g_Megamonas,and g_Parabacteroides was relatively high,and these microbial communities played an important role in maintaining gut microbi-ota homeostasis.After the first chemotherapy,the contribution of c_Betaproteobacteria,o_Burkholderiales,and g_Burkholderia increased,reflecting the effect of chemotherapy on gut microbiota.After the second chemotherapy,the contribu-tion of the f Lactobacillaceae,g_Eubacterium and g_Lactobacillus increased.It was suggested that the repair of the intesti-nal microenvironment was closely related to the recovery of immune regulation,and the dynamic changes of the gut microbiota were closely related to the progression of esophageal cancer and the response to chemotherapy.Conclusion There are signifi-cant differences in the gut microbiota composition between healthy individuals and esophageal cancer patients.The increase in chemotherapy frequency has an effect on the diversity and composition of gut microbiota of esophageal cancer patients.Some mi-crobiota may serve as potential diagnostic markers and therapeutic targets.It provides a new idea for precision medicine of esopha-geal cancer.