不同陈化年份广陈皮对老年脾虚痰湿证大鼠的干预作用及其药效机制

Intervention effects and mechanisms of aged citrus chachiensis hortorum of varying storage years on elderly rats with spleen deficiency and phlegm-dampness syndrome

中文关键词: 陈化年份  脾虚痰湿证  广陈皮  老年大鼠  脂质代谢

英文关键词: aging year  spleen deficiency and phlegm-dampness syndrome  citrus chachiensis hortorum  elderly rat  lipid metabolism

中文摘要:

      目的 探讨不同陈化年份及剂量广陈皮对老年脾虚痰湿证大鼠的干预作用.方法 20月龄SPF级雄性SD大鼠42只,随机分为模型组、对照组、阳性药组、3年高剂量组、3年低剂量组、10年高剂量组和10年低剂量组共7组,每组6只.高脂饲料和不良环境诱导老年大鼠脾虚痰湿证模型.阳性药组给予参苓白术散(1.89 g/kg);3年高/低剂量组、10年高/低剂量组给予相应陈化年份高低剂量广陈皮浓缩液(20 mL/kg),每日灌胃1次.4周后,观察各组大鼠体质量、肝脏湿重、肥胖指数及肝脏系数;检测血清总胆固醇(TC)、三酰甘油(TAG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,肝组织TC、TAG、游离脂肪酸(FFA)水平及环磷酸腺苷(cAMP)/环磷酸鸟苷(cGMP)值.结果 与模型组相比,阳性药组TC、TAG水平降低(P＜0.01);10年高剂量组TC、TAG、LDL-C水平降低,HDL-C水平升高(P＜0.01).与模型组相比,阳性药组、广陈皮各剂量组体质量、肝脏湿重、肥胖指数和肝脏系数下降,TC、TAG、FFA水平降低,cAMP/cGMP值上升(P＜0.05).结论 广陈皮能一定程度改善老年脾虚痰湿证大鼠的脂质代谢紊乱及脂肪肝病变,这一作用可能通过腺苷一磷酸活化蛋白激酶和cAMP-蛋白激酶A信号通路实现.

英文摘要:

      Objective To investigate the intervention effects of citrus chachiensis hortorum(CCH)of different storage years and doses on elderly rats with spleen deficiency and phlegm-dampness syndrome.Methods Forty-two 20-month-old male SPF-grade SD rats were randomly divided into seven groups(n=6 per group):model group,control group,positive drug group,3-year high-dose group,3-year low-dose group,10-year high-dose group,and 10-year low-dose group.A rat model of spleen deficiency and phlegm-dampness syndrome was induced by a high-fat diet and adverse environment.The posi-tive drug group was given Shenling Baizhu powder(1.89 g/kg).The 3-year high/low-dose groups and the 10-year high/low-dose groups were given the corresponding high or low-dose concentrated liquid of CCH(20 mL/kg).The drug was adminis-tered by gavage once a day.After 4 weeks of treatment,the body weight,liver wet weight,obesity index and liver coefficient of the seven groups were observed.The levels of serum total cholesterol(TC),triglycerides(TAG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C),as well as the levels of TC,TAG,free fatty acids(FFA)in liver tissue and the ratio of cyclic adenosine monophosphate(cAMP)/cyclic guanosine monophosphate(cGMP)were detected.Results Compared with the model group,the levels of serum TC and TAG in the positive group significantly decreased(P＜0.01),and the levels of serum TC,TAG,LDL-C in the 10-year high-dose CCH group significantly decreased,but HDL-C level increased(P＜0.01).Compared with the model group,body weight,liver wet weight,obesity index,liver coefficient,and the levels of serum TC,TAG,and hepatic FFA decreased in the positive drug group and various dose CCH groups,while cAMP/cGMP ratio increased significantly(P＜0.05).Conclusion CCH can partially improve lipid metabo-lism disorders and fatty liver lesions in elderly rats with spleen deficiency and phlegm-dampness syndrome.This effect may be achieved through adenosine monophosphate-activated protein kinase and cAMP-protein kinase A signaling pathways.